Enzyme stabilization: choosing the first substitution series
From a public protein and homolog to candidates, WT controls and measurements. What to check before mutagenesis.
2 October 2026 · 5 min readKNOWLEDGE BASE · PROTEIN ENGINEERING
Clear evidence
for your next experiment.
Read predictions, inspect structures and bring measurements back. Practical articles with sources and limits of applicability.
ΔΔG and Tm: why predictions cannot be converted to degrees
Read the ΔΔG sign in Foldiff, distinguish predictions from melting temperature and assess model disagreements.
2 October 2026 · 5 min readpLDDT and PAE: interpreting AlphaFold structure confidence
How pLDDT and PAE differ, what structure confidence means and how Foldiff uses existing AlphaFold DB models.
2 October 2026 · 5 min readpH experiment: from measurements to the observed maximum
Set a pH range, import CSV, interpret means and SD, and choose the next measurement series in Foldiff.
2 October 2026 · 5 min readFireProtDB 2.0: why more data do not make every prediction more accurate
What changed in FireProtDB 2.0 and why measurement type, construct and overlap with model training matter.
2 October 2026 · 5 min read